cd68 e3o7v rabbit monoclonal antibody  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: Female Specific Restrictive Cardiomyopathy and Metabolic Dysregulation in transgenic mice expressing a Peptide of the Amino-Terminus of GRK2

doi: 10.1101/2024.10.14.618348

Figure Lengend Snippet: (A) Representative images of Masson trichrome-stained murine heart sections in non-transgenic littermate control (NLC) and TgβARKnt Sham and TAC mice 4 (left) and 14 (right) weeks after surgery. Quantification of (B) myocyte area and (C) fibrotic area in the ventricles of these mice. Quantification of (D) myocyte area and (E) fibrotic area in the left atria of these mice. *, p < 0.05; **, p < 0.01; ***, p < 0.01; ****, p < 0.0001 by one-way ANOVA with Tukey post-hoc test relative to time-matched NLC Sham. τ , p < 0.05 by one-way ANOVA with Tukey post-hoc test relative to time-matched βARKnt Sham. n = 9-15 hearts per group. (F) Representative images of WGA and 4′,6-diamidino-2-phenylindole (DAPI)-stained murine heart sections from NLC and βARKnt Sham and TAC mice 4 (left) and 14 (right) weeks after surgery. Scale bar, 20 µm. (G) Quantification of cardiomyocyte cross-sectional area (CSA) in these animals. ****, p < 0.0001 by one-way ANOVA with Tukey post-hoc test relative to time-matched NLC Sham. ττττ , p < 0.0001 by one-way ANOVA with Tukey post-hoc test relative to time-matched NLC TAC. n = 489-722 cardiomyocytes from 12-15 hearts per group (∼45 myocytes per heart). Quantification of (H) CD68, (I) CD3, and (J) MPO positive cell area in these mice. *, p < 0.05; **, p < 0.01; ****, p < 0.0001 by one-way ANOVA with Tukey post-hoc test relative to time-matched NLC Sham. n = 3-12 mice per group.

Article Snippet: Next, tissue sections were blocked with 5% Normal Goat Serum (Cell Signaling Technology, CST) in TBST and incubated with rabbit monoclonal CD68 (E3O7V) diluted in SignalStain Antibody Diluent (CST) at 4°C overnight.

Techniques: Staining, Transgenic Assay, Control